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 SARS-COV-2病毒实验室泄漏说的两个新证据 |
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非文人
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作者：非文人 在 驴鸣镇 发贴, 来自 http://www.hjclub.org
SARS-COV-2病毒实验室泄漏说的两个新证据
1. 中国自然科学基金对SARS-Cov-2病毒起源和中间宿主研究的资助是零，而对当初SARS病毒起源和中间宿主研究的资助是近200万。
SARS-2病毒人畜共患自然起源说的主要支持证据就是中间宿主。从利益得失角度看，找到中间宿主的最大受益者是中国，说明病毒灾害来自于自然，而非人禍。中国政府理应投入大量人力物力加强寻找中间宿主的科研工作。
但实际情况如何？以2020年国家自然科研资金分配情况来看，中国政府在寻找SARS-Cov-2病毒中间宿主方面的工作和努力几乎为零。以新冠病毒或SARS-Cov-2病毒为关键词，查寻2020年国家自然科学基金项目，有10项科研资金给与新冠病毒有关研究，但没有关于病毒起源的项目。https://www.medsci.cn/sci/nsfc.do?page=1&txtitle=%E6%96%B0%E5%86%A0%E7%97%85%E6%AF%92&sort_type=3
当初SARS病毒的起源问题，因为世界流行范围较这次SARS-2病毒小很多，其重要性要低得多。但是，国家自然科学基金依然资助了三项有关SARS病毒起源的研究：
1) 我国广东地区SARS病毒来源及基因组特征的研究 （2003年 江丽芳， 30万， 中山大学）
2) 宿主细胞表面SARS病毒Spike蛋白的受体的鉴定（2003年 朱学良， 35万，中国科学院上海生命科学研究院）
3) 应用分子进化和流行病学方法对SARS CoV非自然起源和消失的进一步研究 （2014年 徐德忠， 100万，中国人民解放军第四军医大学）
中国政府对SARS和SARS-2病毒起源研究在资金支持上的强烈反差，不禁让人得出结论：中国高层早已知晓SARS-2病毒不存在中间宿主，不需要白花钱去做无用功。
2. 根据武汉病毒研究所申请瑞德西韦专利的时间，推算武汉病毒研究所分离SARS-COV-2病毒株的时间。
2020年2月4日武汉病毒研究所官网发布消息称2020年1月21日已就瑞德西韦“抗2019新型冠状病毒的用途”申报了中国发明专利。申报依靠的实验数据2020年1月25日送到Cell Research，并发表在2月4日期刊上。https://www.nature.com/articles/s41422-020-0282-0
此论文内容， 包括SARS-COV-2病毒在Vero E6 细胞株中的培养，用抗-蝙蝠似SARS病毒抗体去标识SARS-COV-2病毒，检验七种化合物对SARS-COV-2病毒生长的作用，包括病毒和化合物同时添加以及化合物提前和延迟添加。每种化合物检测了6-7个浓度，每个浓度三个样本，每个浓度都有对照组。病毒感染程度用抗-蝙蝠似SARS病毒抗体, qRT-PCR以及Western Blot分析。这里用抗-蝙蝠似SARS病毒抗体去标识SARS-COV-2病毒就需要大量的预实验，去确定为什么是抗-蝙蝠似SARS病毒抗体而不是抗-人SARS病毒抗体，抗-蝙蝠RaTG13病毒抗体, 或抗-人SARS-COV-2病毒抗体等。
再加上统计分析数据，攥写论文以及英文矫正，以我的经验，一般实验室需要半年到一年时间，最快最厉害的实验室，也需要至少2-3个月的时间来完成此实验研究。
如果武汉病毒研究所用最短的可能时间（2个月）完成的这篇论文，那么，SARS-COV-2病毒株分离的时间就是在2019年11月21日之前，而不是官方公布的2020年1月5日。
英文版：
Two New Pieces of Evidence for the hypothesis of Laboratory leak of SARS-COV-2 Virus
1. The National Natural Science Foundation of China provided zero funding for the research on the origin and intermediate host of the SARS-Cov-2 virus, while the funding for researches on the origin and intermediate host of the SARS virus was nearly 2 million.
The main supporting evidence for the natural origin of the SARS-2 virus is the intermediate host. From the perspective of profit and loss, the biggest beneficiary of finding an intermediate host is China, which shows that the virus disaster comes from nature, not man-made disasters. The Chinese government should invest a lot of manpower and material resources to strengthen scientific research to find intermediate hosts.
But what is the actual situation? Judging from the distribution of national natural science research funds in 2020, the Chinese government's work and efforts in finding an intermediate host of the SARS-Cov-2 virus are almost zero. Using the new coronavirus or SARS-Cov-2 virus as a key word to search for the National Natural Science Foundation of China in 2020, there are 10 scientific research funds for new coronavirus-related research, but there is no project on the origin of the virus. (https://www.medsci.cn/sci/nsfc.do?page=1&txtitle=%E6%96%B0%E5%86%A0%E7%97%85%E6%AF%92&sort_type=3)
On the other hand, the worldwide epidemic of SARS virus was much smaller than this SARS-2 virus, and therefore, the importance of the research on the origin of the SARS virus was much lower. However, the National Natural Science Foundation of China still funded at least three studies on the origin of the SARS virus:
1) Research on the source and genome characteristics of SARS virus in Guangdong, China (2003 Lifang Jiang, 300,000, Sun Yat-sen University)
2) Identification of the receptor for the SARS virus Spike protein on the surface of the host cell (2003 Xueliang Zhu, 350,000, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)
3) Further research on the unnatural origin and disappearance of SARS CoV using molecular evolution and epidemiological methods (2014 Dezhong Xu, 1 million, Fourth Military Medical University of the Chinese People's Liberation Army)
The strong difference between the Chinese government's financial support for the research on the origin of the SARS and SARS-2 viruses has led to the conclusion that Chinese top officials have long known that there is no intermediate host for the SARS-2 virus, and there is no need to spend money to do useless work.
2. According to the time when the Wuhan Institute of Virology applied for the patent of Remdesivir, the time for the Wuhan Institute of Virology to isolate the SARS-COV-2 strain was estimated.
On February 4, 2020, the Wuhan Institute of Virology announced on its official website that on January 21, 2020, it had declared a Chinese invention patent on the "use of anti-2019 novel coronavirus" of Remdesivir. The experimental data that the application relies on was sent to Cell Research on January 25, 2020, and published in the February 4th journal. https://www.nature.com/articles/s41422-020-0282-0
The content of this paper includes the culture of SARS-COV-2 virus in Vero E6 cell line, the use of anti-bat SARS-like virus antibodies to identify SARS-COV-2 virus, and the test of seven compounds on the growth of SARS-COV-2 virus. The experiments include the simultaneous addition of viruses and compounds, the early or delayed addition of compounds. Each compound was tested at 6-7 concentrations, with three samples for each concentration, and a control group for each concentration. The degree of virus infection was analyzed by anti-bat SARS-like virus antibody, qRT-PCR and Western Blot. Using anti-bat-like SARS virus antibodies to identify SARS-COV-2 virus here requires a lot of preliminary experiments to determine why anti-bat-like SARS virus antibodies instead of others such as anti-human SARS virus antibodies, anti-bat RaTG13 virus, and anti-human SARS-2 virus antibody, etc.
In addition to statistical analysis of data, writing papers, and English corrections, in my experience, the general laboratory may need half a year to a year, and the fastest and most powerful laboratory may also need at least 2-3 months to complete this experimental study.
If the Wuhan Institute of Virology completed this paper in 2 months, then the time for the isolation of the SARS-COV-2 virus strain would be before November 21, 2019, instead of the officially announced January 5, 2020.
作者：非文人 在 驴鸣镇 发贴, 来自 http://www.hjclub.org
上一次由非文人于2021-6-28 周一, 上午2:20修改，总共修改了4次 |
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